Cryopreservation makes it possible to preserve eggs, sperm and embryos for further use in fertility treatment programs. Frozen material can remain intact for years, and this gives cryo technology many possibilities. The most effective and safe method of cryopreservation of embryos and germ cells is ultrafast freezing called vitrification. This method avoids the main danger of freezing, the formation of ice crystals that can damage cells during thawing.



Cryopreservation of Embryos

During the procedure of in vitro fertilization, it is possible to obtain more embryos than suggested for transfer. In such cases, the cryopreservation and storage of embryos services can be performed at the request of the patients.

 After incubation in special media, the embryo "saturated" with cryo protectants is placed on a carrier, which is quickly immersed in liquid nitrogen. The carrier contains the basic data of the patient. In this cold and comfortable "medium", the embryo can wait for years to awaken.

When the “X” time comes, the embryo is thawed. The thawing process involves the unfreezing embryo by washing it out of cryo-holder using special liquid and then “dragging” thru solutions, allowing the cryoprotectants to yield to the water instead. And there you have got alive, healthy and strong embryo which is ready for transfer!

Modern cryo methods are very effective – more than 95% of all of the vitrified embryos being successfully thawed. The pregnancy rates using cycles of cryopreserved embryos are equal or even exceeding the same indices of cycles using fresh embryos.

Cryopreservation of Oocytes

Vitrification is the most advanced method of cryopreservation that successfully used to preserve oocytes. However, it is essential to keep in mind that women’s cells are vulnerable at the freezing process, and consequently the efficiency of vitrification of oocytes is slightly lower than the results of embryo vitrification.

  • women who are faced with functional impairment or subject to ovaries removal, to give a birth to a biological child in the future;
  • patients diagnosed with cancer – prior to chemo and x-ray therapy, as it leads to deprivation of the reproductive cells quality;
  • women who need to delay the moment of getting pregnant for whatever reasons without losing the reproductive potential.

Sperm Cryopreservation

Nonetheless, the important component of the assisted reproductive technologies is the sperm cryopreservation. Sperm cells are quite hardy and tolerant to freezing and thawing, which allows using thawed sperm for in vitro fertilization.

 • if a man has to undergo difficult treatment or when a man works in hazardous industries;

• if the partner cannot be physically present at the clinic on the day of IVF or play the role of a “fallback” when the semen analysis does not meet the requirements.

Please, note that the cryopreservation process does not affect to the health of the unborn baby, however, it is likely that the cold season would be its favorite time of a year.